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dylight-649-conjugated donkey anti-rabbit  (Jackson Immuno)


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    Structured Review

    Jackson Immuno dylight-649-conjugated donkey anti-rabbit

    Dylight 649 Conjugated Donkey Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight-649-conjugated donkey anti-rabbit/product/Jackson Immuno
    Average 90 stars, based on 1 article reviews
    dylight-649-conjugated donkey anti-rabbit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Identifying new players in structural synaptic plasticity through dArc1 interrogation"

    Article Title: Identifying new players in structural synaptic plasticity through dArc1 interrogation

    Journal: iScience

    doi: 10.1016/j.isci.2023.108048


    Figure Legend Snippet:

    Techniques Used: Recombinant, Illumina Sequencing, Magnetic Beads, SYBR Green Assay, Software



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    Dock180 interacts with GRASP in live cells. A split YFP system was used to demonstrate the interaction of Dock180 and GRASP in cells. Dock180 and GRASP were respectively labeled with the N and C-terminal halves of venus (VN-Dock180 or VC-GRASP). Neither VN nor VC is fluorescent by itself. YFP fluorescence is reconstituted if the two fusion partners interact, thereby bringing VN and VC into close proximity so that a functional YFP can be formed. MDCK cells were transfected with the indicated constructs using Neon® (1400, 20, 2). They were then plated on Fibronectin coated coverslips. After 18 hours, Cells were fixed and stained with mouse anti-myc (cytohesin 2) or mouse anti- Flag (Dock180) followed by Dylight™ 594- conjugated anti-mouse secondary antibody and rabbit anti-HA (GRASP) followed by <t>Dylight™649-</t> conjugated anti-rabbit secondary antibody. In the merge cytohesin 2 or Dock180 are psuedocolored red, GRASP is psuedocolored blue, and the YFP channel detecting Venus indicating that the 2 expressed proteins interact is pseudocolored green. Bar, 10 μm.
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    Image Search Results


    Journal: iScience

    Article Title: Identifying new players in structural synaptic plasticity through dArc1 interrogation

    doi: 10.1016/j.isci.2023.108048

    Figure Lengend Snippet:

    Article Snippet: DyLight-649-conjugated donkey anti-rabbit , Jackson ImmunoResearch , Cat#711495152.

    Techniques: Recombinant, Illumina Sequencing, Magnetic Beads, SYBR Green Assay, Software

    Dock180 interacts with GRASP in live cells. A split YFP system was used to demonstrate the interaction of Dock180 and GRASP in cells. Dock180 and GRASP were respectively labeled with the N and C-terminal halves of venus (VN-Dock180 or VC-GRASP). Neither VN nor VC is fluorescent by itself. YFP fluorescence is reconstituted if the two fusion partners interact, thereby bringing VN and VC into close proximity so that a functional YFP can be formed. MDCK cells were transfected with the indicated constructs using Neon® (1400, 20, 2). They were then plated on Fibronectin coated coverslips. After 18 hours, Cells were fixed and stained with mouse anti-myc (cytohesin 2) or mouse anti- Flag (Dock180) followed by Dylight™ 594- conjugated anti-mouse secondary antibody and rabbit anti-HA (GRASP) followed by Dylight™649- conjugated anti-rabbit secondary antibody. In the merge cytohesin 2 or Dock180 are psuedocolored red, GRASP is psuedocolored blue, and the YFP channel detecting Venus indicating that the 2 expressed proteins interact is pseudocolored green. Bar, 10 μm.

    Journal: BMC Cell Biology

    Article Title: The scaffolding protein GRASP/Tamalin directly binds to Dock180 as well as to cytohesins facilitating GTPase crosstalk in epithelial cell migration

    doi: 10.1186/1471-2121-14-9

    Figure Lengend Snippet: Dock180 interacts with GRASP in live cells. A split YFP system was used to demonstrate the interaction of Dock180 and GRASP in cells. Dock180 and GRASP were respectively labeled with the N and C-terminal halves of venus (VN-Dock180 or VC-GRASP). Neither VN nor VC is fluorescent by itself. YFP fluorescence is reconstituted if the two fusion partners interact, thereby bringing VN and VC into close proximity so that a functional YFP can be formed. MDCK cells were transfected with the indicated constructs using Neon® (1400, 20, 2). They were then plated on Fibronectin coated coverslips. After 18 hours, Cells were fixed and stained with mouse anti-myc (cytohesin 2) or mouse anti- Flag (Dock180) followed by Dylight™ 594- conjugated anti-mouse secondary antibody and rabbit anti-HA (GRASP) followed by Dylight™649- conjugated anti-rabbit secondary antibody. In the merge cytohesin 2 or Dock180 are psuedocolored red, GRASP is psuedocolored blue, and the YFP channel detecting Venus indicating that the 2 expressed proteins interact is pseudocolored green. Bar, 10 μm.

    Article Snippet: DyLight™ 649 conjugated donkey anti-rabbit as well as DyLight™ 594 conjugated donkey anti mouse were purchased from Jackson ImmunoResearch laboratories (West Grove, PA).

    Techniques: Labeling, Fluorescence, Functional Assay, Transfection, Construct, Staining